TY - JOUR
T1 - Sperm chromatin stability and their relationship with fertilization rate in Sheep of the Junín race
AU - Unchupaico-Payano, Ide
AU - Alponte-Sierra, Alberto
AU - Quispe-Eulogio, Carlos
AU - Ancco-Goméz, Edith
AU - La Cruz, Alex Huamán De
AU - Mariño-Alfaro, Julio
AU - Patiño-Rivera, Alberto
AU - Lavado-Meza, Carmencita
AU - Huanca-Rojas, Lupe
AU - Bazán-Alonso, Luis
N1 - Publisher Copyright:
© (2022). All Rights Reserved.
PY - 2022
Y1 - 2022
N2 - The objective of this research was to evaluate the effect of sperm on chromatin stability and its relationship with the membrane integrity structural – physiological and the rate of fertilization of female sheep. Ejaculates of sperm (2 × 109 sperm·mL-1) with 70% of motility were collected using an artificial vagina (n=5, 2 years old. For this, each ram was served with fifteen female sheep (n=75), generating thus five different Groups (A, B, C, D, and E). A control Group also was considered. Sperm nuclear chromatin stability (NCS) was evaluated using the Borate Buffer (BB), Sodium Dodecyl Sulfate (SDS), and the mixture of Ethylenediaminetetraacetic acid (EDTA) + SDS. The fertilization rate was evaluated after 16-18 hours post sperm injection. Sperm concentration showed a significant difference (P<0.05) between Groups. In Contrast, seminal volume, and sperm motility do not show a significant difference (P>0.05). A high correlation (r2=0.52) was observed between morphology and motility, and the fertilization rate was 74.6% (n=56). It was concluded in general that techniques to evaluate nuclear condensation values do have a high likelihood to give a diagnosis about the future potential of sperm populations in Junín ram.
AB - The objective of this research was to evaluate the effect of sperm on chromatin stability and its relationship with the membrane integrity structural – physiological and the rate of fertilization of female sheep. Ejaculates of sperm (2 × 109 sperm·mL-1) with 70% of motility were collected using an artificial vagina (n=5, 2 years old. For this, each ram was served with fifteen female sheep (n=75), generating thus five different Groups (A, B, C, D, and E). A control Group also was considered. Sperm nuclear chromatin stability (NCS) was evaluated using the Borate Buffer (BB), Sodium Dodecyl Sulfate (SDS), and the mixture of Ethylenediaminetetraacetic acid (EDTA) + SDS. The fertilization rate was evaluated after 16-18 hours post sperm injection. Sperm concentration showed a significant difference (P<0.05) between Groups. In Contrast, seminal volume, and sperm motility do not show a significant difference (P>0.05). A high correlation (r2=0.52) was observed between morphology and motility, and the fertilization rate was 74.6% (n=56). It was concluded in general that techniques to evaluate nuclear condensation values do have a high likelihood to give a diagnosis about the future potential of sperm populations in Junín ram.
KW - Peruvian rams
KW - Sperm chromatin
KW - membrane integrity
UR - http://www.scopus.com/inward/record.url?scp=85172872888&partnerID=8YFLogxK
U2 - 10.52973/rcfcv-e32157
DO - 10.52973/rcfcv-e32157
M3 - Original Article
AN - SCOPUS:85172872888
SN - 0798-2259
VL - 32
JO - Revista Cientifica de la Facultad de Veterinaria
JF - Revista Cientifica de la Facultad de Veterinaria
M1 - e32157
ER -