TY - JOUR
T1 - Biophenolic compounds and metal ions associated with the antioxidant and antibacterial activity of the ethanolic extract of Heliotropium arborescens L. leaves from the Andean region of Ayacucho-Peru
AU - Alvarado, Angel T.
AU - Chávez, Haydee
AU - García, Jorge A.
AU - Bendezú, María R.
AU - Surco-Laos, Felipe
AU - Molina-Cabrera, Aura
AU - Laos-Anchante, Doris
AU - Vega-Ramos, Nelly
AU - Palomino-Jhong, Juan J.
AU - Yarasca-Carlos, Paulina Eliades
AU - Loja-Herrera, Berta
AU - Pineda-Pérez, Mario
AU - Bolarte-Arteaga, Mario
N1 - Publisher Copyright:
Copyright Alvarado AT et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC-BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
PY - 2024
Y1 - 2024
N2 - Heliotropium arborescens (Galán de Mera et al. 2022) is a herbaceous plant that contains various biophenolic compounds that exhibit antibacterial property and antioxidant activity that could prevent diseases caused by free radicals. The objective was to study the biophenolic compounds and metal ions associated with the in vitro antioxidant and antibacterial activity of the ethanolic extract of Heliotropium arborescens L. leaves from the Andean region of Ayacucho-Peru. The total polyphenolic content (TPC) was determined by the Folin-Ciocalteu method; Total flavonoid content (TFC) was determined by spectrophotometry at 510 nm, TFC was expressed in mg quercetin/g sample (mg QE/g). Antioxidant activity by three in vitro methods; determination of metals by flame atomic absorption spectrophotometry (FAAS) and antibacterial activity by agar diffusion method. The values obtained from the TPC were 306.80±0.05 mg GAE/g, TFC 111.53±0.25 mg QE/g. Antioxidant activity: DPPH IC50 37.55±0.04 µL and 65.64±0.10 µL, at 0.47 and 0.93 mg/mL extract, respectively; ABTS 0.20±0.01 mM TEAC/g (0.41 mg/mL extract) and 0.47±0.01 (0.82 mg/mL extract); FRAP 0.24±0.01 mM TEAC/g (0.94 mg/mL extract) and 0.46±0.02 mM TEAC/g (1.88 mg/mL extract). Correlation relationship TFC/DPPH (r=0.9994; R2 0.9989), TFC/ABTS (r = 0.9572; R2 0.9998) and TFC/FRAP (r = 09933; R2 0.9868). Calcium 1.22±0.04, copper 0.77±0.05, magnesium 851.7±3.06 and zinc 20.63±1.19 mg/100 g were found in all cases. Antibacterial activity on Bacillus subtilis (ATCC 6633) and Staphylococcus aureus (ATCC 25923) with a minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) > 400 μg/mL. It is concluded that the total biophenolic components and metals of the ethanolic extract of the leaves of H. arborescens L. show potential antioxidant activity, and moderate antibacterial activity against Bacillus subtilis and Staphylococcus aureus.(Figure
AB - Heliotropium arborescens (Galán de Mera et al. 2022) is a herbaceous plant that contains various biophenolic compounds that exhibit antibacterial property and antioxidant activity that could prevent diseases caused by free radicals. The objective was to study the biophenolic compounds and metal ions associated with the in vitro antioxidant and antibacterial activity of the ethanolic extract of Heliotropium arborescens L. leaves from the Andean region of Ayacucho-Peru. The total polyphenolic content (TPC) was determined by the Folin-Ciocalteu method; Total flavonoid content (TFC) was determined by spectrophotometry at 510 nm, TFC was expressed in mg quercetin/g sample (mg QE/g). Antioxidant activity by three in vitro methods; determination of metals by flame atomic absorption spectrophotometry (FAAS) and antibacterial activity by agar diffusion method. The values obtained from the TPC were 306.80±0.05 mg GAE/g, TFC 111.53±0.25 mg QE/g. Antioxidant activity: DPPH IC50 37.55±0.04 µL and 65.64±0.10 µL, at 0.47 and 0.93 mg/mL extract, respectively; ABTS 0.20±0.01 mM TEAC/g (0.41 mg/mL extract) and 0.47±0.01 (0.82 mg/mL extract); FRAP 0.24±0.01 mM TEAC/g (0.94 mg/mL extract) and 0.46±0.02 mM TEAC/g (1.88 mg/mL extract). Correlation relationship TFC/DPPH (r=0.9994; R2 0.9989), TFC/ABTS (r = 0.9572; R2 0.9998) and TFC/FRAP (r = 09933; R2 0.9868). Calcium 1.22±0.04, copper 0.77±0.05, magnesium 851.7±3.06 and zinc 20.63±1.19 mg/100 g were found in all cases. Antibacterial activity on Bacillus subtilis (ATCC 6633) and Staphylococcus aureus (ATCC 25923) with a minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) > 400 μg/mL. It is concluded that the total biophenolic components and metals of the ethanolic extract of the leaves of H. arborescens L. show potential antioxidant activity, and moderate antibacterial activity against Bacillus subtilis and Staphylococcus aureus.(Figure
KW - antibacterial
KW - antioxidant
KW - biophenols
KW - flavonoids
KW - Heliotropium arborescens
UR - http://www.scopus.com/inward/record.url?scp=85195314945&partnerID=8YFLogxK
U2 - 10.3897/PHARMACIA.71.E120315
DO - 10.3897/PHARMACIA.71.E120315
M3 - Original Article
AN - SCOPUS:85195314945
SN - 0428-0296
VL - 71
SP - 1
EP - 12
JO - Pharmacia
JF - Pharmacia
ER -