TY - JOUR
T1 - Anti HSV-1 activity of halistanol sulfate and halistanol sulfate C isolated from Brazilian marine sponge Petromica citrina (Demospongiae)
AU - Guimarães, Tatiana Da Rosa
AU - Quiroz, Carlos Guillermo
AU - Rigotto, Caroline
AU - De Oliveira, Simone Quintana
AU - De Almeida, Maria Tereza Rojo
AU - Bianco, Éverson Miguel
AU - Moritz, Maria Izabel Goulart
AU - Carraro, João Luís
AU - Palermo, Jorge Alejandro
AU - Cabrera, Gabriela
AU - Schenkel, Eloir Paulo
AU - Reginatto, Flávio Henrique
AU - Simões, Cláudia Maria Oliveira
PY - 2013/11
Y1 - 2013/11
N2 - The n-butanol fraction (BF) obtained from the crude extract of the marine sponge Petromica citrina, the halistanol-enriched fraction (TSH fraction), and the isolated compounds halistanol sulfate (1) and halistanol sulfate C (2), were evaluated for their inhibitory effects on the replication of the Herpes Simplex Virus type 1 (HSV-1, KOS strain) by the viral plaque number reduction assay. The TSH fraction was the most effective against HSV-1 replication (SI = 15.33), whereas compounds 1 (SI = 2.46) and 2 (SI = 1.95) were less active. The most active fraction and these compounds were also assayed to determine the viral multiplication step(s) upon which they act as well as their potential synergistic effects. The anti-HSV-1 activity detected was mediated by the inhibition of virus attachment and by the penetration into Vero cells, the virucidal effect on virus particles, and by the impairment in levels of ICP27 and gD proteins of HSV-1. In summary, these results suggest that the anti-HSV-1 activity of TSH fraction detected is possibly related to the synergic effects of compounds 1 and 2.
AB - The n-butanol fraction (BF) obtained from the crude extract of the marine sponge Petromica citrina, the halistanol-enriched fraction (TSH fraction), and the isolated compounds halistanol sulfate (1) and halistanol sulfate C (2), were evaluated for their inhibitory effects on the replication of the Herpes Simplex Virus type 1 (HSV-1, KOS strain) by the viral plaque number reduction assay. The TSH fraction was the most effective against HSV-1 replication (SI = 15.33), whereas compounds 1 (SI = 2.46) and 2 (SI = 1.95) were less active. The most active fraction and these compounds were also assayed to determine the viral multiplication step(s) upon which they act as well as their potential synergistic effects. The anti-HSV-1 activity detected was mediated by the inhibition of virus attachment and by the penetration into Vero cells, the virucidal effect on virus particles, and by the impairment in levels of ICP27 and gD proteins of HSV-1. In summary, these results suggest that the anti-HSV-1 activity of TSH fraction detected is possibly related to the synergic effects of compounds 1 and 2.
KW - Antiviral activity
KW - HSV-1
KW - Marine sponge
KW - Petromica citrina
KW - Sulfate sterols
UR - http://www.scopus.com/inward/record.url?scp=84889259587&partnerID=8YFLogxK
U2 - 10.3390/md11114176
DO - 10.3390/md11114176
M3 - Original Article
C2 - 24172213
AN - SCOPUS:84889259587
SN - 1660-3397
VL - 11
SP - 4176
EP - 4192
JO - Marine Drugs
JF - Marine Drugs
IS - 11
ER -