Validación de la cromatografía líquida de alto rendimiento acoplada a espectrometría de masas para determinar albendazol sulfóxido en plasma humano

Jaime Rosales-Rimache, Saúl Santiváñez, Luis Moreno-Exebio, Miguel Grande, María Flores-Rodríguez, María Eguiluz, Hugo García

Research output: Contribution to journalOriginal Articlepeer-review

Abstract

Context: Albendazole is used in the treatment of cysticercosis and hydatid disease, and its prolonged administration can generate adverse effects attributed to the active metabolite, albendazole sulfoxide (ABZSO), whose measurement requires validated methods that help monitor patients under treatment. Aims: To validate a high-performance liquid chromatography method with mass detection (HPLC-MS) for the determination of ABZSO in human plasma. Methods: HPLC-MS was performed on C18 columns (100 × 4.6 mm × 3.5 µm) and mobile phase composed of methanol, water and ammonia solution (25%) (800:200:0.1 v /v/v) at a flow rate of 0.5 mL/minute. The calibration curve was built with an albendazole standard, and detection in a triple quadrupole system. Results: The method was linear (1-1500 ng/mL, R2 = 0.999) with a detection limit of 1 ng/mL. ABZSO concentrations at 1, 5, 50, 100, 200, 500, 1000, and 1500 ng/mL had relative standard deviations of 10.4, 8.4, 7.8, 2.9, 7.4, 3.2, 3.4 and 1.3%. It was highly precise with a mean coefficient of variation of 1.411%, and accurate with a bias of -14.7; 2.8 and -0.8% in ABZSO concentrations of 5, 500 and 1000 ng/mL. The average recovery was 89.2%, and the stability of ABZSO after refrigeration had an average coefficient of variation of 2.3%. Conclusions: The HPLC-MS method is valid and reliable to determine ABZSO, and could be used in pharmacokinetic, bioequivalence, and other studies that evaluate the biological monitoring of patients receiving oral treatment with albendazole.

Translated title of the contributionValidation of high performance liquid chromatography coupled to mass spectrometry to determine albendazole sulfoxide in human plasma
Original languageAmerican English
Pages (from-to)888-895
Number of pages8
JournalJournal of Pharmacy and Pharmacognosy Research
Volume10
Issue number5
DOIs
StateIndexed - Sep 2022
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2022 Journal of Pharmacy & Pharmacognosy Research.

Keywords

  • HPLC
  • albendazole
  • analytical validity

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