Sperm chromatin stability and their relationship with fertilization rate in Sheep of the Junín race

Ide Unchupaico-Payano, Alberto Alponte-Sierra, Carlos Quispe-Eulogio, Edith Ancco-Goméz, Alex Huamán De La Cruz, Julio Mariño-Alfaro, Alberto Patiño-Rivera, Carmencita Lavado-Meza, Lupe Huanca-Rojas, Luis Bazán-Alonso

Research output: Contribution to journalOriginal Articlepeer-review


The objective of this research was to evaluate the effect of sperm on chromatin stability and its relationship with the membrane integrity structural – physiological and the rate of fertilization of female sheep. Ejaculates of sperm (2 × 109 sperm·mL-1) with 70% of motility were collected using an artificial vagina (n=5, 2 years old. For this, each ram was served with fifteen female sheep (n=75), generating thus five different Groups (A, B, C, D, and E). A control Group also was considered. Sperm nuclear chromatin stability (NCS) was evaluated using the Borate Buffer (BB), Sodium Dodecyl Sulfate (SDS), and the mixture of Ethylenediaminetetraacetic acid (EDTA) + SDS. The fertilization rate was evaluated after 16-18 hours post sperm injection. Sperm concentration showed a significant difference (P<0.05) between Groups. In Contrast, seminal volume, and sperm motility do not show a significant difference (P>0.05). A high correlation (r2=0.52) was observed between morphology and motility, and the fertilization rate was 74.6% (n=56). It was concluded in general that techniques to evaluate nuclear condensation values do have a high likelihood to give a diagnosis about the future potential of sperm populations in Junín ram.

Translated title of the contributionEstabilidad de la cromatina espermática y su relación con la tasa de fecundación en ovejas de la raza Junín
Original languageAmerican English
Article numbere32157
JournalRevista Cientifica de la Facultad de Veterinaria
StateIndexed - 2022
Externally publishedYes

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  • Peruvian rams
  • Sperm chromatin
  • membrane integrity


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