Pilot study of COBAS PCR and ligase chain reaction for detection of rectal infections due to Chlamydia trachomatis

Aldo Lucchetti; Jorge Sanchez; Connie L. Celum; William L.H. Whittington; King K. Holmes

doi:10.1128/JCM.41.5.2174-2175.2003

Pilot study of COBAS PCR and ligase chain reaction for detection of rectal infections due to Chlamydia trachomatis

Aldo Lucchetti, Jorge Sanchez, Connie L. Celum, William L.H. Whittington, King K. Holmes

Research output: Contribution to journal › Original Article › peer-review

15 Scopus citations

Abstract

We tested rectal specimens from men who have sex with men for Chlamydia trachomatis by using COBAS PCR (Roche Diagnostics) and ligase chain reaction LCR (Abbott laboratories) and compared three PCR specimen-processing procedures. Chlamydiae were detected by one or more procedures in 22 of 186 specimens. All three PCR tests were positive for 17 specimens, all of which also tested positive by LCR.

Original language	American English
Pages (from-to)	2174-2175
Number of pages	2
Journal	Journal of Clinical Microbiology
Volume	41
Issue number	5
DOIs	https://doi.org/10.1128/JCM.41.5.2174-2175.2003
State	Indexed - 1 May 2003
Externally published	Yes

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10.1128/JCM.41.5.2174-2175.2003

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title = "Pilot study of COBAS PCR and ligase chain reaction for detection of rectal infections due to Chlamydia trachomatis",

abstract = "We tested rectal specimens from men who have sex with men for Chlamydia trachomatis by using COBAS PCR (Roche Diagnostics) and ligase chain reaction LCR (Abbott laboratories) and compared three PCR specimen-processing procedures. Chlamydiae were detected by one or more procedures in 22 of 186 specimens. All three PCR tests were positive for 17 specimens, all of which also tested positive by LCR.",

author = "Aldo Lucchetti and Jorge Sanchez and Celum, \{Connie L.\} and Whittington, \{William L.H.\} and Holmes, \{King K.\}",

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AU - Lucchetti, Aldo

AU - Sanchez, Jorge

AU - Celum, Connie L.

AU - Whittington, William L.H.

AU - Holmes, King K.

PY - 2003/5/1

Y1 - 2003/5/1

N2 - We tested rectal specimens from men who have sex with men for Chlamydia trachomatis by using COBAS PCR (Roche Diagnostics) and ligase chain reaction LCR (Abbott laboratories) and compared three PCR specimen-processing procedures. Chlamydiae were detected by one or more procedures in 22 of 186 specimens. All three PCR tests were positive for 17 specimens, all of which also tested positive by LCR.

AB - We tested rectal specimens from men who have sex with men for Chlamydia trachomatis by using COBAS PCR (Roche Diagnostics) and ligase chain reaction LCR (Abbott laboratories) and compared three PCR specimen-processing procedures. Chlamydiae were detected by one or more procedures in 22 of 186 specimens. All three PCR tests were positive for 17 specimens, all of which also tested positive by LCR.

UR - https://www.scopus.com/pages/publications/0038627926

U2 - 10.1128/JCM.41.5.2174-2175.2003

DO - 10.1128/JCM.41.5.2174-2175.2003

M3 - Original Article

C2 - 12734272

AN - SCOPUS:0038627926

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JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

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ER -

Pilot study of COBAS PCR and ligase chain reaction for detection of rectal infections due to Chlamydia trachomatis

Abstract

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