Local and systemic effects of BtaMP-1, a new weakly hemorrhagic Snake Venom Metalloproteinase purified from Bothriopsis taeniata Snake Venom

Frank Denis Torres-Huaco; Silvana Maruñak; Pamela Teibler; Soledad Bustillo; Ofelia Acosta de Pérez; Laura Cristina Leiva; Luis Alberto Ponce-Soto; Sergio Marangoni

doi:10.1016/j.ijbiomac.2019.09.032

Local and systemic effects of BtaMP-1, a new weakly hemorrhagic Snake Venom Metalloproteinase purified from Bothriopsis taeniata Snake Venom

Frank Denis Torres-Huaco, Silvana Maruñak, Pamela Teibler, Soledad Bustillo, Ofelia Acosta de Pérez, Laura Cristina Leiva, Luis Alberto Ponce-Soto, Sergio Marangoni

Research output: Contribution to journal › Original Article › peer-review

3 Scopus citations

Abstract

A new weak hemorrhagic metalloproteinase named BtaMP-1 was purified from Bothriopsis taeniata snake venom by molecular exclusion followed by anion exchange chromatographies. This protein showed a molecular mass of 25,968.16 Da and is composed of 218 amino acid residues. The multiple alignments of its partial amino acid sequence showed high structural identity with other P-I class SVMP. BtaMP-1 showed caseinolytic activity that was enhanced by Ca²⁺ ion, completely inhibited by chelating and reducing agents and can be classified as an α-fibrinogenolytic enzyme. Locally, BtaMP-1 induces hemorrhage and edema, but not myotoxicity. These findings were confirmed by histological analysis of mouse gastrocnemius muscle. “In vitro” studies suggest that BtaMP-1 induce cytotoxicity in myoblast C2C12 but not in the myotubes cell line. BtaMP-1 induced systemic alterations in mice with one MHD and two hours exposure; histological analysis of lungs showed hemorrhagic areas, congestion, and increase the thickness of alveolar septum. Also, this protein induced mild effects on kidney and disruption of coagulation by depletion of fibrinogen plasma levels. This work provides insights into the importance of BtaMP-1 biological effects in envenomation by Bothropsis taeniata snake venom and providing further evidence to understand the role of P-I class SVMP in ophidian envenomation.

Original language	American English
Pages (from-to)	1044-1054
Number of pages	11
Journal	International Journal of Biological Macromolecules
Volume	141
DOIs	https://doi.org/10.1016/j.ijbiomac.2019.09.032
State	Indexed - 1 Dec 2019
Externally published	Yes

Bibliographical note

Funding Information:
This work was supported by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) - Finance Code 001 , and was part of the doctoral thesis of Frank Denis Torres Huaco.

Funding Information:
The author's thanks to Mr. Paulo A. Baldasso for technical assistance, to Prof. Dr. Stephen Hyslop for reviewing and correcting the English language, and the Mass Spectrometry Laboratory at Brazilian Biosciences National Laboratory, CNPEM/ABTLUS, Campinas, Brazil, for its support with the MS analyses. This work was supported by the Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - Brasil (CAPES) - Finance Code 001, and was part of the doctoral thesis of Frank Denis Torres Huaco.

Publisher Copyright:
© 2019 Elsevier B.V.

Keywords

Bothriopsis taeniata
Cytotoxicity
Lung hemorrhage

Access to Document

10.1016/j.ijbiomac.2019.09.032

Cite this

Torres-Huaco, F. D., Maruñak, S., Teibler, P., Bustillo, S., Acosta de Pérez, O., Leiva, L. C., Ponce-Soto, L. A., & Marangoni, S. (2019). Local and systemic effects of BtaMP-1, a new weakly hemorrhagic Snake Venom Metalloproteinase purified from Bothriopsis taeniata Snake Venom. International Journal of Biological Macromolecules, 141, 1044-1054. https://doi.org/10.1016/j.ijbiomac.2019.09.032

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title = "Local and systemic effects of BtaMP-1, a new weakly hemorrhagic Snake Venom Metalloproteinase purified from Bothriopsis taeniata Snake Venom",

abstract = "A new weak hemorrhagic metalloproteinase named BtaMP-1 was purified from Bothriopsis taeniata snake venom by molecular exclusion followed by anion exchange chromatographies. This protein showed a molecular mass of 25,968.16 Da and is composed of 218 amino acid residues. The multiple alignments of its partial amino acid sequence showed high structural identity with other P-I class SVMP. BtaMP-1 showed caseinolytic activity that was enhanced by Ca2+ ion, completely inhibited by chelating and reducing agents and can be classified as an α-fibrinogenolytic enzyme. Locally, BtaMP-1 induces hemorrhage and edema, but not myotoxicity. These findings were confirmed by histological analysis of mouse gastrocnemius muscle. “In vitro” studies suggest that BtaMP-1 induce cytotoxicity in myoblast C2C12 but not in the myotubes cell line. BtaMP-1 induced systemic alterations in mice with one MHD and two hours exposure; histological analysis of lungs showed hemorrhagic areas, congestion, and increase the thickness of alveolar septum. Also, this protein induced mild effects on kidney and disruption of coagulation by depletion of fibrinogen plasma levels. This work provides insights into the importance of BtaMP-1 biological effects in envenomation by Bothropsis taeniata snake venom and providing further evidence to understand the role of P-I class SVMP in ophidian envenomation.",

keywords = "Bothriopsis taeniata, Cytotoxicity, Lung hemorrhage",

author = "Torres-Huaco, {Frank Denis} and Silvana Maru{\~n}ak and Pamela Teibler and Soledad Bustillo and {Acosta de P{\'e}rez}, Ofelia and Leiva, {Laura Cristina} and Ponce-Soto, {Luis Alberto} and Sergio Marangoni",

note = "Funding Information: This work was supported by the Coordena{\c c}{\~a}o de Aperfei{\c c}oamento de Pessoal de N{\'i}vel Superior - Brasil (CAPES) - Finance Code 001 , and was part of the doctoral thesis of Frank Denis Torres Huaco. Funding Information: The author's thanks to Mr. Paulo A. Baldasso for technical assistance, to Prof. Dr. Stephen Hyslop for reviewing and correcting the English language, and the Mass Spectrometry Laboratory at Brazilian Biosciences National Laboratory, CNPEM/ABTLUS, Campinas, Brazil, for its support with the MS analyses. This work was supported by the Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - Brasil (CAPES) - Finance Code 001, and was part of the doctoral thesis of Frank Denis Torres Huaco. Publisher Copyright: {\textcopyright} 2019 Elsevier B.V.",

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Torres-Huaco, FD, Maruñak, S, Teibler, P, Bustillo, S, Acosta de Pérez, O, Leiva, LC, Ponce-Soto, LA & Marangoni, S 2019, 'Local and systemic effects of BtaMP-1, a new weakly hemorrhagic Snake Venom Metalloproteinase purified from Bothriopsis taeniata Snake Venom', International Journal of Biological Macromolecules, vol. 141, pp. 1044-1054. https://doi.org/10.1016/j.ijbiomac.2019.09.032

Local and systemic effects of BtaMP-1, a new weakly hemorrhagic Snake Venom Metalloproteinase purified from Bothriopsis taeniata Snake Venom. / Torres-Huaco, Frank Denis; Maruñak, Silvana; Teibler, Pamela et al.
In: International Journal of Biological Macromolecules, Vol. 141, 01.12.2019, p. 1044-1054.

Research output: Contribution to journal › Original Article › peer-review

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AU - Torres-Huaco, Frank Denis

AU - Maruñak, Silvana

AU - Teibler, Pamela

AU - Bustillo, Soledad

AU - Acosta de Pérez, Ofelia

AU - Leiva, Laura Cristina

AU - Ponce-Soto, Luis Alberto

AU - Marangoni, Sergio

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N2 - A new weak hemorrhagic metalloproteinase named BtaMP-1 was purified from Bothriopsis taeniata snake venom by molecular exclusion followed by anion exchange chromatographies. This protein showed a molecular mass of 25,968.16 Da and is composed of 218 amino acid residues. The multiple alignments of its partial amino acid sequence showed high structural identity with other P-I class SVMP. BtaMP-1 showed caseinolytic activity that was enhanced by Ca2+ ion, completely inhibited by chelating and reducing agents and can be classified as an α-fibrinogenolytic enzyme. Locally, BtaMP-1 induces hemorrhage and edema, but not myotoxicity. These findings were confirmed by histological analysis of mouse gastrocnemius muscle. “In vitro” studies suggest that BtaMP-1 induce cytotoxicity in myoblast C2C12 but not in the myotubes cell line. BtaMP-1 induced systemic alterations in mice with one MHD and two hours exposure; histological analysis of lungs showed hemorrhagic areas, congestion, and increase the thickness of alveolar septum. Also, this protein induced mild effects on kidney and disruption of coagulation by depletion of fibrinogen plasma levels. This work provides insights into the importance of BtaMP-1 biological effects in envenomation by Bothropsis taeniata snake venom and providing further evidence to understand the role of P-I class SVMP in ophidian envenomation.

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KW - Bothriopsis taeniata

KW - Cytotoxicity

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ER -

Local and systemic effects of BtaMP-1, a new weakly hemorrhagic Snake Venom Metalloproteinase purified from Bothriopsis taeniata Snake Venom

Abstract

Bibliographical note

Keywords

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