Field performance of a rapid diagnostic test for the serodiagnosis of abdominal cystic echinococcosis in the peruvian highlands

Tommaso Manciulli, Raul Enríquez-Laurente, Francesca Tamarozzi, Raffaella Lissandrin, Maira Elizalde, Cesar Sedano, Karina Bardales, Ambra Vola, Annalisa de Silvestri, Carmine Tinelli, Enrico Brunetti, Saul Santivanez, Mara Mariconti

Research output: Contribution to journalOriginal Articlepeer-review

5 Scopus citations

Abstract

We evaluated the performance of a commercial rapid diagnostic test (RDT) in a field setting for the diagnosis of abdominal cystic echinococcosis (CE) using sera collected during an ultrasound population screening in a highly endemic region of the Peruvian Andes. Abdominal CE was investigated by ultrasonography. Sera collected from individuals with abdominal CE (cases) and age- and gender-matched volunteers with no abdominal CE (controls) were tested independently in two laboratories (Peru and Italy) using the VIRapid® HYDATIDOSIS RDT and RIDASCREEN® Echinococcus IgG enzyme-linked immunosorbent assay. Performance indexes of single and serially combined tests were calculated and applied to hypothetical screening and clinical scenarios. Test concordance was also evaluated. Prevalence of abdominal CE was 6.00% (33 of 546) by ultrasound. Serum was obtained from 33 cases and 81 controls. The VIRapid test showed similar sensitivity (76% versus 74%) and lower specificity (79% versus 96%) than results obtained in a hospital setting. RDTs showed better performance when excluding subjects reporting surgery for CE and if weak bands were considered negative. Concordance between tests was moderate to very good. In hypothetical screening scenarios, ultrasound alone or confirmed by RDTs provided more reliable prevalence figures than serology alone, which overestimated it by 5 to 20 times. In a simulation of case diagnosis with pre-test probability of CE of 50%, positive and negative post-test probabilities of the VIRapid test were 78% and 22%, respectively. The application of the VIRapid test alone would not be reliable for the assessment of population prevalence of CE, but could help clinical decision making in resource-limited settings.

Original languageAmerican English
Pages (from-to)181-187
Number of pages7
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume105
Issue number1
DOIs
StateIndexed - Jul 2021
Externally publishedYes

Bibliographical note

Publisher Copyright:
Copyright © 2021 by The American Society of Tropical Medicine and Hygiene

Fingerprint

Dive into the research topics of 'Field performance of a rapid diagnostic test for the serodiagnosis of abdominal cystic echinococcosis in the peruvian highlands'. Together they form a unique fingerprint.

Cite this